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Sorbitol-induced nuclear-cytoplasmic redistribution of Cgs1p. (A) Fluorescence photomicrographs of cgs1-GFP (Cgs1-GFP) and pka1-GFP (Pka1-GFP) cells cultured to mid-log phase in YEAU containing 2 M sorbitol. (B) The cgs1-GFP strain was cultured in YEAU to 2 × 106 cells/ml and then diluted with an equal volume of YEAU containing 4 M sorbitol for a final concentration of 2 M sorbitol. The culture was then examined by fluorescence microscopy at the indicated time points.
