FIG. 6.

Halo assay of MTLa strains overexpressing CAG1. Cells in the opaque phase were spread onto an SD plate. The black dots indicate the positions where 5 μl of either the α-factor synthetic peptide (1 μg/μl; α) or the solvent, as a negative control (control), was spotted. The plates are shown after 2 days of incubation at 24°C. Halos delineate the zones of growth arrest induced by α-factor. The overexpression of the CAG1 gene (o/e CAG1) suppresses the sensitivity of the Δsst2 strain to the pheromone. wt, wild type.