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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Bioconjug Chem. 2007 Dec 7;19(1):377–384. doi: 10.1021/bc700315v

Figure 1. Effect of dithionite treatment and pH on NBD fluorescence emission intensity.

Figure 1

(A) Fluorescence emission of NBD-labeled oligos and lipid- and polymer-based gene carriers incorporating NBD-labeled oligos was measured in the presence and absence of dithionite. Values are presented as the percentage fluorescence emission compared to an equivalent amount of uncomplexed, unquenched NBD-oligo ± standard deviation. (B) Fluorescence emission of NBD-oligos was measured in acidic, neutral, and basic buffers. Values are presented as the percentage fluorescence emission compared to an equivalent amount of NBD-oligo in PBS (pH 7.4) ± standard deviation.