Skip to main content
. 1998 Aug;9(8):2185–2200. doi: 10.1091/mbc.9.8.2185

Figure 2.

Figure 2

Figure 2

Disruption of adherens junctions by HGF/SF is blocked by the MAPKK1/2 inhibitor PD098059 and the PI 3-kinase inhibitor LY294002. (A) MDCK cells were stimulated with HGF/SF for 4 h (b and f) or were preincubated with 50 μM PD098059 (c and g) or 20 μM LY294002 (d and h) before addition of HGF/SF for 4 h. MDCK cells were then fixed and stained for β-catenin (a–d) and E-cadherin (e–h) localization. Bar, 10 μm. (B) MDCK cells were treated as indicated with 20 μM LY294002 (LY) or 50 μM PD098059 (PD) before addition of HGF/SF (HGF) for 4 h. Disrupted junctions in control cells (cont) and stimulated cells were defined as junctions where no E-cadherin and β-catenin could be detected at the sites of intercellular contact and were subsequently counted. The numbers represent the mean of 300 cells, which were counted in three independent experiments.