Figure 3.

E-cadherin and β-catenin are redistributed from the NP-40-insoluble fraction to the soluble fraction in response to HGF/SF. Cell lysates of unstimulated control cells (C) or cells incubated with HGF/SF for 4 h (HGF) or cells treated with 20 μM LY 294002 for 1 h before addition of HGF/SF (HGF/LY) or 50 μM PD98059 (HGF/PD) were fractionated into a NP-40-soluble (S) and NP-40-insoluble (I) fraction. Equal sample volumes were loaded and probed for E-cadherin (top blot) and β-catenin (bottom blot).