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HGF/SF induces sustained activation of p42/p44 MAPK. Cells were stimulated with HGF/SF (HGF) for 10 min or 4 or 16 h with (+) or without (−) preincubation with 50 μM PD098059. The cells were subsequently lysed, and the lysates were electrophoresed on an SDS-gel. The top panel shows Western blotting for the activated, phosphorylated form of p42/p44 MAPK (pMAPK). The bottom panel shows the blot reprobed with an anti-ERK1 antibody against total p42/p44 MAPK (ERK1/ERK2).
