Figure 5.

Dominant negative mutants of MAPKK1 block disruption of adherens junctions. MDCK cells were microinjected with a construct expressing a dominant negative mutant of MAPKK1, AFG MAPKK1 (100 ng/μl). After microinjection cells were incubated for 6 h before addition of HGF/SF for 2 h. Cells were stained for β-catenin (a) and for expression of the mutant MAPKK1 using the rabbit anti-MAPKK1 antibody (b). The arrow in a shows β-catenin localizing to adherens junctions in injected cells, and the arrowhead indicates disrupted adherens junctions in uninjected cells. Bar, 10 μm.