Table 4.
Synthetic lethality between temperature-sensitive spc110 alleles and spc97 and spc98 alleles
| Location of mutationsa | Allele | spc97-62 | spc97-113 | spc97-114 | spc98-63 | |
|---|---|---|---|---|---|---|
| N terminus | Region I | spc110-221 | SLb | SL | SL | SL |
| Region I | spc110-222 | SL | SL | SL | SL | |
| C terminus | Region II | spc110-226 | NO | NO | SL | NO |
| Region III | spc110-220 | NO | NO | SL | NO | |
| Regions II + III | spc110-225 | NO | SL | SL | NO | |
| Truncation | spc110-224 | SL | NO | SL | NO | |
a
Mutations in the spc110 alleles fall into three regions: region I is residues 1-163 N-terminal to the coiled-coil domain. Region II is aa 772-836. Region III is aa 897-917 and is the calmodulin-binding site.
b
SL, Synthetic lethality is detected between the two alleles at 30°C. NO, Double mutants are able to sector at 30°C. Genetic analysis is described in MATERIALS AND METHODS. The diploid strains that were sporulated to obtain these results are listed in Table 1.