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AP Release from CCV is dependent on cytosol and ATP. CCV were incubated without (lanes 1 and 3) or with (lane 2 and 4) 65 μg of bovine brain cytosol; and with (lanes 3 and 4) or without (lanes 1 and 2) 800 μM ATP with a regenerating system. Hexokinase and glucose were included in samples without ATP. Released APs, present in the supernatant after centrifugation, were detected on Western blots with polyclonal antibody 0927.
