Figure 2.

Cytosol titration into AP release assay. (A) CCVs (3.75 μg) were incubated in the presence of an ATP-regenerating system with increasing amounts of cytosol in the absence (open circle) or presence (closed circle) of 0.5 μg of hsc70 or with increasing amounts of hsc70 in the absence of cytosol (closed triangle) for 5 min. at 25°C. AP release from CCV was detected by ELISA after centrifugation at 4°C, as described in MATERIALS AND METHODS. Hsc70 concentration is indicated by the lower abscissa and is equivalent to the amount of hsc70 present in the corresponding amount of cytosol.