Figure 4.

AP release requires Hsc70. (A) CCV were incubated with or without hsc70 as indicated: without additional cytosol (panel 1), with 65 μg of complete cytosol (panel 2), or with 65 μg of hsc70-depleted cytosol (panel 3). AP release (solid bars) was detected by ELISA, and clathrin release (hatched bars) was detected by densitometry of Coomassie- stained SDS-PAGE gels. (B) AP release was measured by ELISA after incubation of CCV with hsc70-depleted cytosol in the absence (open squares) and presence (closed circles) of 0.5 μg of hsc70. Error bars are hidden by the symbol in some cases.