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ATP dependence of AP and clathrin release. CCV were incubated with 65 μg cytosol, hsc70, an ATP-regenerating system, and the indicated amount of ATP. Clathrin release (closed circle, as determined by densitometry of Coomassie-stained gels) and AP release (open triangle, as determined by ELISA) were measured for each sample. Inset shows a double-reciprocal plot for ATP dependence of AP release. Error bars are hidden by the symbol in some cases.
