Table 1.
Nucleotide effects in the presence of a regenerating system
| Treatment | Clathrin release | AP release |
|---|---|---|
| Control | [100] | [100] |
| +ATPγS | 81.1 ± 18.0 | 97 ± 11.1 |
| +AMP-PNP | 73.9 ± 12.9 | 90.7 ± 6.4 |
| +ADPβS | 66.6 ± 2.4 | 121.8 ± 6.2 |
| +GTP | 114.2 ± 2.6 | 97.6 ± 2.0 |
| +GTPγS | 83.9 ± 4.5 | 107.0 ± 13.4 |
| +3C5 | 6.8 ± 11.7 | 71.7 ± 15.8 |
AP and clathrin release were measured, as described in MATERIALS AND METHODS, after incubation of CCV with 65 μg cytosol, hsc70, 50 μM ATP, and ATP-regenerating system (control conditions); 1 mM of each nucleotide or nonhydrolyzable nucleotide analog was included in the reactions as indicated. Both AP and clathrin release are normalized to control conditions; results are shown ±SE.