Table 2.

Nucleotide effects in the absence of a regenerating system

Treatment	Clathrin release	AP release
Control (ATP regenerating)	[100]	[100]
2 mM ATP	100.8 ± 6.7	107.0 ± 12.7
2 mM ATPγS	71.1 ± 4.6	72.0 ± 1.25
2 mM AMP-PNP	12.2 ± 0.9	16.5 ± 0.24

The release of APs and clathrin from CCV was measured, as described in Table 1, after incubation of CCV with 50 μg cytosol, 1 μg hsc70, and 2 mM of the indicated nucleotide or nonhydrolyzable nucleotide analog, or 800 μM ATP with a regenerating system (control condition). AP and clathrin release are normalized to control conditions.