Skip to main content
. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: J Proteome Res. 2007 Oct 26;6(12):4711–4719. doi: 10.1021/pr700571d

Figure 3.

Figure 3

HIPK2 modulates HMGA1a’s binding affinity towards human germ line promoter. (a) EMSA of HMGA1a (control, lanes 2-4), HIPK2-phosphorylated HMGA1a (lanes 6-8) and HMGA1a in the presence of HIPK2 but not ATP (lanes 9-11). “A1a” and “pA1a_HIPK2” represent recombinant and HIPK2-phosphorylated HMGA1a proteins, respectively. (b) Quantification results based on triplicate measurements of the control recombinant HMGA1a (in shaded bar) and HMGA1a in the presence of HIPK2 (in black bar) at different protein concentrations. For the EMSA results of pA1a_HIPK2 shown in lanes 9-11, HIPK2 was also present in the binding reaction mixture.