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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: J Proteome Res. 2007 Oct 26;6(12):4711–4719. doi: 10.1021/pr700571d

Figure 4.

Figure 4

(a) EMSA for the binding of recombinant HMGA1a (lanes 1-2), cdc2-phosphorylated HMGA1a (lanes 3-4) and HIPK2-phosphorylated HMGA1a (lanes 5-6) to human germ line ε promoter. (b) Quantitative results from triplicate measurements from (a) of HMGA1a (“A1a” in white bar), HIPK2-phosphorylated HMGA1a (“pA1a_HIPK2” in black bar) and cdc2-phosphorylated HMGA1a (“pA1a_cdc2” in shaded bar) at different protein concentrations. To exclude the interference introduced by the presence of either HIPK2 or cdc2, these kinases were removed prior to carrying out the EMSA experiments.