Skip to main content
. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: J Proteome Res. 2007 Nov 23;7(1):328–338. doi: 10.1021/pr070300g

Figure 3.

Figure 3

Purity of the mitochondrial preparation. 3 μg of protein from each fraction during purification was subjected to electrophoresis. Proteins were transferred to PVDF membrane and probing with antibodies directed against tubulin as a cytoskeletal marker and prohibitin as a mitochondrial marker. Lanes contained the following fractions: 1. Total homogenate; 2. Post-nuclear supernatant; 3. Nuclear pellet; 4. Post-mitochondrial supernatant; 5. Crude mitochondrial pellet; 6. Microsomal fraction; and 7. Purified mitochondria.