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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: J Proteome Res. 2007 Nov 23;7(1):328–338. doi: 10.1021/pr070300g

Figure 5.

Figure 5

Immunoblots validate changes in expression of mitochondrial proteins during differentiation. 3 μg samples of total cell proteins from cultures at different stages in the induction program from day 0 through 11 were subjected to SDS-PAGE, blotted and probed for a variety of mitochondrial proteins. Antibodies used were specific for individual polypeptides of the respiratory complexes I–IV, GAPDH (loading control), Porin (VDAC), Cyt. C (cytochrome C), AIF (apoptosis inducing factor), PHB (prohibitin 1), MnSOD (manganese superoxide dismutase), and HSP 60 (heat shock protein 60).