Table 2. Comparison of abundance changes for 14 mitochondrial proteins observed in both the total cell protein and the mitochondrial fraction.
The relative abundance of 14 mitochondrial proteins for NLC relative to undifferentiated cells is shown for total cell lysates and for the purified mitochondrial fraction. The right-hand column shows the ratio of total cell to mitochondrial protein data.
| Accession | Protein Name | Gene | iTRAQ® Cell | Abundance Mito | Data Cell/Mito |
|---|---|---|---|---|---|
| NP_031531 | F1 ATPase alpha | Atp5a1 | 1.861 | 1.137 | 1.637 |
| NP_058054 | F1 ATPase beta | Atp5b | 1.770 | 0.951 | 1.861 |
| NP_035824 | VDAC 1 | Vdac1 | 2.034 | 1.193 | 1.705 |
| NP_079843 | cytochrome c-1 | Cyc1 | 1.313 | 0.695 | 1.888 |
| NP_079986 | Complex III, Rieske Fe-S 1 | Uqcrfs1 | 1.786 | 1.050 | 1.701 |
| NP_034607 | HSP 60 | Hsp60 | 0.845 | 0.636 | 1.328 |
| NP_034611 | HSP 70 | GRP75 | 1.016 | 0.654 | 1.554 |
| NP_032329 | HSP 10 | Hspe1 | 0.846 | 0.506 | 1.673 |
| NP_031557 | Prohibitin 2 | BAP | 1.170 | 0.686 | 1.706 |
| NP_032643 | MDH 2 | MDH | 1.751 | 1.154 | 1.518 |
| NP_080720 | Citrate synthase | Cis | 1.911 | 1.275 | 1.499 |
| NP_542364 | aconitase 2 | Aco2 | 2.332 | 1.533 | 1.521 |
| NP_031477 | Ant 2 | Slc25a5 | 1.242 | 0.844 | 1.472 |
| NP_598429 | Phosphate carrier | Phc | 1.523 | 0.986 | 1.543 |
| Average | 1.615 |