Table 3. Differential expression of mRNAs regulated upon NLC differentiation.
Quantitative mRNA analysis of cycle threshold (Ct) changes of Phgdh (internal control), PGC1α, PRC1, and mybbp1a for undifferentiated P19 cells day 0, 4, and 11. (S.D. = standard deviation).
| Relative Ct Values
|
Relative to Day 0
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene | Day 0 | S.D.
+/− |
Day 4 | S.D
+/− |
Day 11 | S.D
+/− |
Δ Ct
Day 4 |
Δ Ct
Day 11 |
Fold Δ
Day 4 |
Fold Δ
Day 11 |
|
| ||||||||||
| PHGDH | 19.83 | 0.30 | 18.67 | 0.27 | 22.54 | 0.10 | −1.16 | 2.71 | 2.24 | 0.15 |
| PGC1α | 30.21 | 0.33 | 30.95 | 0.23 | 22.93 | 0.09 | 0.74 | −7.28 | 0.60 | 155.88 |
| PGC1β | 30.07 | 0.24 | 28.06 | 0.20 | 26.31 | 0.11 | −2.01 | −3.75 | 4.03 | 13.49 |
| PRC1 | 23.77 | 0.32 | 22.26 | 0.51 | 23.52 | 0.22 | −1.51 | −0.25 | 2.85 | 1.19 |
| Mybbp1a | 20.10 | 0.19 | 20.78 | 0.41 | 21.99 | 0.49 | 0.68 | 1.89 | 0.63 | 0.27 |