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Journal of Virology logoLink to Journal of Virology
. 1985 Mar;53(3):1001–1004. doi: 10.1128/jvi.53.3.1001-1004.1985

Purification of simian virus 40 large T antigen by immunoaffinity chromatography.

R A Dixon, D Nathans
PMCID: PMC254743  PMID: 2983081

Abstract

Simian virus 40 large T antigen from lytically infected cells has been purified to near homogeneity by immunochromatography of the cell extract on a protein A-Sepharose-monoclonal antibody column. The resulting T antigen retains biochemical activity; i.e., it hydrolyzes ATP and binds to simian virus 40 DNA at the origin of replication.

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Selected References

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