TABLE 4.

FRET parameters for the decameric cyclic cationic peptide GS10WY at 25°C

Environment	QD	JDA (M−1 cm−1 nm4)	R0 (Å)	r (Å)	FRET efficiency
Buffer	0.039	3.034 × 1012	11.4	7.2	0.94
80% Methanol	0.027	4.067 × 1012	11.2	12.6	0.33
POPC vesicles	0.033	2.072 × 1012	10.7	13.3	0.21

For description and calculation of parameters, see the Materials and Methods and Results sections. Peptide concentrations for FRET measurements were 10 μM. The P/L ratio for the cyclic peptides in POPC lipid dispersions was 1:100; at this ratio, all peptides were bound to vesicles, as verified by CD concentration-dependent experiments (e.g., Fig. 3 A).