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Gel-filtration studies on oligomerization of ecarpholin S induced by suramin. (A) Elution profiles of ecarpholin S without suramin. Column is Superdex 200 (Amersham Biosciences). (B) Elution profiles of ecarpholin S with suramin (ecarpholin S/suramin = 1:1, molar ratio). (C) Elution profiles of ecarpholin S with suramin. (ecarpholin S/suramin = 1:2, molar ratio). (D) Column calibrated with gel-filtration standard (1.35–670 kDa from Bio-Rad Laboratories, Hercules, California.
