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Dependence of the recombinant human carbonic anhydrase II melting temperature on the total added concentration of EZA (□) and TFMSA (▴). Dashed lines represent the fit assuming KbU → 0, Eq. 15, whereas solid lines are fit using Eq. 13. For vanishing 
the fitted 
constants have values of 
and 
M−1 for EZA and TFMSA, respectively. The binding constants (under the condition when 
was allowed to vary) are as follows: 
and 
M−1 for EZA, and 
and 
M−1 for TFMSA.
