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Dependence of the Plk1-PBD melting temperature on the concentration of added peptide in two forms: phosphorylated (▪) and unphosphorylated (▵). Lines are drawn according to the model of Eq. 13, using the following parameters: 
and 
For phosphorylated peptide, the binding constant was 
M−1. The unphosphorylated peptide binding constant was nondetectable (
M−1).
