Figure 2.

Lack of suppressive activity and absence of an anergic state by CB CD25⁺CD4⁺ T cells. CD4⁺CD25⁺ and CD4⁺CD25⁻ T cells were purified by a magnetic microbead system. (a) Cells were lysed and immunoblotted with anti-FOXP3 Ab (top panel) or anti-β-actin Ab (bottom panel). (b) Cells were stimulated with anti-CD3/CD28-mAb-coated beads under the indicated conditions. Cell proliferation was assessed by ³H-thymidine uptake on day 4. Data are representative of 5 different donors.