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. Author manuscript; available in PMC: 2009 Aug 15.
Published in final edited form as: Cancer Res. 2008 Aug 15;68(16):6851–6858. doi: 10.1158/0008-5472.CAN-08-0718

Figure 3. Thrombocytopenia reduces cancer cell proliferation and locally affects tumor viability.

Figure 3

At day 8 after subcutaneous implantation of LLC tumors, thrombocytopenia was induced and 48 hours later, tumors were harvested and sectioned. A. Mice were injected with BrdUrd 3 hours before sacrifice and the proliferative index was calculated as the percentage of BrdUrd positive nuclei relative to DAPI-stained nuclei (n = 10 microscopic fields out of 4 tumors for each). B. The apoptotic index was calculated as the percentage of TUNEL positive nuclei relative to DAPI-stained nuclei (n = 10 microscopic fields out of 5 tumors for each). C. H&E, DAPI and TUNEL staining of non-hemorrhagic and hemorrhagic areas of the LLC tumors. Arrows indicate fragmented and condensed nuclei. Bars = 20 μm.