Fig. 5.
Effect of EGF family of growth factors on phosphorylation of the EGFR. Goblet cells from primary culture were stimulated with EGF (10−7 M), TGF-α (10−7 M) or HB-EGF (10−7 M) for 5 min. Cells were scraped and analyzed via Western blot with antibodies against phosphorylated EGFR (Tyr1068) or total EGFR. A representative blot is shown in A. Data shown in B are mean ± SEM from 4 independent experiments. * indicates p < 0.05.