Effect of EGF family of growth factors on signaling intermediates. Goblet cells from primary culture were stimulated with EGF (10−7 M), TGF-α (10−7 M), HB-EGF (10−7 M), and heregulin (10−7 M) for 5 min. Cells were scraped and multiplex analysis was performed for activation of Erk, JNK, p38 MAPK, and AKT. Data are mean ± SEM from 5 independent experiments. * indicates p < 0.05. Here – heregulin.