Table 2.
Information on the antibody reagents; details on the specificity controls are described in the Materials and Methods.
| Target | Antigen | Species | Dilution | Source |
|---|---|---|---|---|
| Calbindin D28k | Recombinant calbindin D28k | Rabbit
AB1778 |
1:1000 | Chemicon |
| CTIP2 | human CTIP2 fusion protein | Rat
AB18465 |
1:1000 | Abcam |
| Calretinin | Recombinant rat calretinin | Rabbit
AB5054 |
1:2500 | Chemicon |
| Calretinin | Recombinant human calretinin | Rabbit
7699/4 |
1:4000 | Swant |
| Cleaved Caspase-3 | RGTELDCGIETD | Rabbit
#9664 |
1:400
1:200 |
Cell Signaling |
| ChAT | Human placental ChAT | Goat
AB144P |
1:250 | Chemicon |
| DLX2 | purified MBP-DLX2 fusion protein (aa:1-154) | Guinea pig | 1:2000 | Kazuaki Yoshikawa |
| Pan-DLX | N-terminal 200 aa + protein (aa:1-154) butterfly DLX protein | Rabbit | 1:250 | Grace Panganiban |
| Rabbit GFP | GFP isolated directly from Aequorea Victoria | Rabbit
A11122 |
1:1000 | Invitrogen |
| Chicken GFP | purified recombinant GFP | Chicken
GFP-1020 |
1:1000 | Aves lab. |
| NPAS1 | Mouse NPAS1 peptide (aa: 139-594) | Rabbit | 1:1000 | Steve McKnight |
| NPY | Synthetic porcine NPY | Rabbit
N9528 |
1:5000 | Sigma |
| NPY | synthetic porcine NPY | Rabbit
22940 |
1:2000 | Immunostar |
| PV | Purified frog muscle PV | Mouse
P 3088 |
1:2000 | Sigma |
| PH3 | Human histone H3 peptide (aa: 7-20) | Rabbit
06-570 |
1:500 | Upstate |
| PLAP | Purified human PLAP | Rabbit
AHP537HT |
1:50 | AbD Serotec |
| SS | Synthetic 1-14 cyclic SS | Rat
MAB354 |
1:150 | Chemicon |
| TBR1 | TBR1 peptide (aa:1-20) | Rabbit | 1:1000 | Robert Hevner |