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Detection of Ste14p in whole cell lysates. Crude yeast cell extracts (0.4 OD600 cell equivalents) were resolved by 12.5% SDS-PAGE and transferred to nitrocellulose. Ste14p was detected using rabbit polyclonal anti-Ste14p antibodies that were depleted previously of nonspecific antibodies (described in MATERIALS AND METHODS). Lane 1, SM2926 (Δste14-3); lane 2, SM3041 (chromosomal STE14); lane 3, SM3185 (CEN STE14); and lane 4, SM3495 (2μ STE14).
