Figure 4.

Transient transfection of rat Cx43 increases transcriptional activity of OC and BSP promoters in UMR 106/01 and MC3T3-E1 cells. Cells were cotransfected with either a rat Cx43 expression construct or its vector, and one of the promoter–luciferase constructs, OCLUC, BSPLUC or SV40LUC, as indicated. Luciferase activity was measured 3 d after transfection. Data are representative of four (UMR 106–01) and six (MC3T3-E1) different experiments and are expressed as the average ± SD of triplicate wells for each condition. *, p < 0.01 vs. vector-transfected cells (t test for unpaired samples).