Figure 5.

Effect of coexpression of ROCK and mDia on the actin cytoskeleton in MDCK cells. pCAG-myc-ROCK-Δ1 plus pEF-BOS-myc-mDia-ΔN were microinjected into the nuclei of MDCK cells. At 10 h after the microinjection, the cells were fixed and double stained with rhodamine-phalloidin (a and b) and the anti-myc mAb (c) or triple stained with rhodamine-phalloidin (d), the anti-vinculin mAb (e), and the anti-myc pAb (f). (a, d, e, and f) Confocal microscopic analysis at the basal levels. (b and c) Confocal microscopic analysis at the junctional levels. The results shown are representative of three independent experiments. Bars, 10 μm.