Figure 2.

Sucrose gradient fractionation analysis of PC12 cells. Postnuclear supernatants from control cells and Csp1 overexpressing clone 1 were loaded onto a 0.6–1.8 M linear sucrose gradient and centrifuged at 100,000 × g for 6 h at 4°C. Fractions were collected, diluted in SDS sample buffer, separated by SDS-PAGE on 12.5% gels, and blotted onto nitrocellulose. The blots were then probed with specific antisera. Immunoreactivity of fractions was quantified by densitometry and plotted as a percentage of the most immunoreactive fraction. (A) Characterization of synaptophysin, syntaxin, and secretogranin II (SGII) in fractions from control cells. (B) Csp distribution in control cells and overexpressing clone 1.