Figure 4.

Csp overexpression has no observable effect on intracellular Ca²⁺ signals. Cells were loaded with the Ca²⁺-sensitive dye Fura-2 and depolarized by addition of 50 mM KCl while monitoring cell fluorescence at 340 and 380 nm. The basal [Ca²⁺]_i and the peak and sustained increases in Ca²⁺ concentration after depolarization were calculated. (A) Representative traces from control cells and overexpressing clones 1 and 2. (B) Averaged data from 12 experiments, expressed as mean ± SEM.