Figure 5.

Regulated exocytosis is enhanced from cells overexpressing Csp. Cells in 24-well trays (0.5 × 10⁶/well) were incubated with [³H]dopamine for 90 min at 37°C. The cells were washed, permeabilized for 6 min with 20 μM digitonin, and challenged by addition of 0 or 10 μM Ca²⁺ (A) or 100 μM GTPγS in the absence of Ca²⁺ (B), and release of [³H]dopamine was determined. Data are expressed as mean ± SEM of the percentage of total dopamine cell content (n = 6). For GTPγS the data are shown with basal release at 0 Ca²⁺ subtracted.