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. 1999 Aug;10(8):2493–2506. doi: 10.1091/mbc.10.8.2493

Figure 1.

Figure 1

Treatment of EGFR-CD533 and EGFR-antisense cells with doxycycline increases expression of EGFR-CD533 and reduces expression of EGFR, respectively. EGFR-CD533 (A) and EGFR-antisense (B) cells were cultured as in MATERIALS AND METHODS and were treated with 1 μg/ml doxycycline for 48 h before experimentation. After 48 h, cells were lysed, and equal total protein amounts (100 μg) were immunoprecipitated, followed by immunoblotting with the same antibody to determine the expression of EGFR and EGFR-CD533. Exposure of A was for 2 min; exposure of B was for 60 s. Representative experiments for EGFR-CD533 (n = 5) and EGFR-antisense (n = 3) cells are shown.