Figure 10.

FAK^F/V associates with Fyn and induces tyrosine phosphorylation of paxillin. (A) CE cells transfected with the RCAS A vector (lane 1) and cells expressing wild-type FAK (lane 2) or FAK^F/V (lane 3) were lysed, and endogenous Fyn was immunoprecipitated using a polyclonal antibody. Coimmunoprecipitating FAK or FAK^F/V was detected by Western blotting using BC2. (B) CE cells transfected with the RCAS A vector (lane 1), cells expressing exogenous Fyn (lane 2), wild-type FAK (lane 3), or FAK^F/V alone (lane 5), or cells coexpressing Fyn and FAK (lane 4) or Fyn and FAK^F/V (lane 6) were lysed, and paxillin was immunoprecipitated. The immune complexes were analyzed by Western blotting for phosphotyrosine (top) and for paxillin (bottom). The position of the 68-kDa molecular weight marker is indicated by the arrowheads. I.P., Immunoprecipitate.