Figure 3.

The FAT sequence is required for integrin-dependent regulation. Cells expressing epitope-tagged wild-type FAK (lanes 1–3), tagged dl 853–963 (lanes 4–6), or tagged dl 965–1012 (lanes 7–9) were analyzed. Exogenously expressed, tagged FAK was immunoprecipitated from lysates of cultured cells (lanes 1, 4, and 7) or cells plated on poly-l-lysine–coated plates (lanes 2, 5, and 8) or fibronectin-coated plates (lanes 3, 6, and 9) using the KT3 antibody. The immune complexes were analyzed by Western blotting for phosphotyrosine (top) and for the amount of protein present using KT3 (bottom). The position of the 97-kDa molecular weight marker is indicated by the arrowheads.