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. Author manuscript; available in PMC: 2008 Sep 23.
Published in final edited form as: Biochemistry. 2006 Apr 25;45(16):5251–5260. doi: 10.1021/bi0521590

Figure 3. N-Glycosydase F treatment.

Figure 3

100 μg of membranes were incubated with 3 μl PNGase F overnight at 37 ° C followed by SDS/PAGE separation and immunoblotting with the BXP-21 monoclonal anti-ABCG2 antibody, resulting in a clear shift to a smaller molecular weight band in the wild type ABCG2 transfected and the flavopiridol selected MCF-7 control cell lines (MCF-7/FLV1000), while there is now significant shift in case of the G553L mutant.