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. Author manuscript; available in PMC: 2008 Sep 23.
Published in final edited form as: Biochemistry. 2006 Apr 25;45(16):5251–5260. doi: 10.1021/bi0521590

Figure 6. The G553L mutant in Sf9 insect cells.

Figure 6

A: Wild type ABCG2 and the G553L mutant are detectable on the surface of Sf9 cells with the 5D3 monoclonal anti-ABCG2 antibody on flow cytometry (as detailed in Figure 2). B: The G553L mutant displays similar basal ATPase activity as the non-functional K86M mutant in Sf9 membranes. ATPase activity was measured determining the liberation of inorganic phosphate from ATP with a colorimetric reaction, Ko143 is a specific inhibitor of ABCG2.