Skip to main content
. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Dev Biol. 2008 Jun 9;321(1):64–76. doi: 10.1016/j.ydbio.2008.05.555

Figure 4.

Figure 4

Spry1;Col2 embryos have reduced bone formation. (A) Von Kossa staining of long bone sections at E16.5 (50X). (B) Higher magnification from rectangular area of A. Note the persistence of hypertrophic chondrocytes and failure of formation of normal trabecular bone. (C) in situ hybridization for osteocalcin showed inhibition of osteogenesis in Spry1;Col2 growth plates. (D, E) Alcian Blue staining of E16.5 humerus sections. (D), higher magnification of D (E). Chondrocytes in CAG-GFP-Spry1 control mice formed an orderly columnar zone (black bracket), but the same region in Spry1;Col2-Cre mice was highly disorganized. R=resting, P=proliferating, H=hypertrophic zones.