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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Dev Biol. 2008 Jun 9;321(1):64–76. doi: 10.1016/j.ydbio.2008.05.555

Figure 5.

Figure 5

Overexpression of Spry1 in chondrocytes delayed chondrocyte differentiation and maturation resulting in accumulation of hypertrophic cartilage. (A-L) In situ hybridization for stage-specific chondrocyte differentiation markers on sections of E16.5 humeri from CAG-GFP-Spry1 control and Spry1;Col2-Cre embryos. The reserve chondrocyte marker aggrecan (Agc) (A, B), the prehypertrophic and hypertrophic chondrocyte marker Indian hedgehog (Ihh) (C, D), PTHrP receptor (Ppr) (E, F), hypertrophic chondrocyte marker Col10α1 (G, H, I, J), and the terminal differentiated marker MMP-13 (K, L) were employed. In situ hybridization was carried out on E13.5 forelimb sections from control (M, N) and Spry1;Col2-Cre (O, P) for Ihh (M, O) and Col10α1 (N, P). LacZ staining of forelimbs (Q) and hindlimbs (R) of ROSA26;Col2-Cre (WT) and Spry1;ROSA26;Col2-Cre (Spry1) embryos.