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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Dev Biol. 2008 Jun 9;321(1):64–76. doi: 10.1016/j.ydbio.2008.05.555

Figure 6.

Figure 6

Overexpression of Spry1 in chondrocytes inhibited cell proliferation. (A, B) Short-term (2hr) BrdU incorporation into E16.5 mouse embryos shows the rate of chondrocyte proliferation. Immunochemistry showed decreased BrdU-labeled cells in the region of the presumptive proliferating columnar chondrocytes (P) of Spry1;Col2-Cre mice with little difference in the reserve chondrocytes (R) (A). Quantification of BrdU-positive cells in Spry1;Col2-Cre sections relative to controls (B). (C, D) Tamoxifen(TM)-induced Spry1 expression in primary chondrocytes inhibits cell growth. (C) Immunoblot analysis demonstrates tamoxifen-induced Spry1 protein expression. (D) Cell growth assay with and without tamoxifen-induced Spry1 expression in primary chondrocytes from CAG-GFP-Spry1;CAGGCre-ERTM embryos. Assays were performed in triplicate.