. Author manuscript; available in PMC: 2008 Sep 23.

Published in final edited form as: Biomacromolecules. 2007 Feb;8(2):477–484. doi: 10.1021/bm0605863

Table 2.

Effects of Lys₃, Lys₄, Lys_5, and poly-L-lysine on the melting T_m of λ-DNA

Trilysine (Lys₃)		Tetralysine (Lys₄)		Pentalysine (Lys₅)
Conc., μM	T_m,°C	Conc., μM	T_m, °C	Conc., μM	T_m, °C
0	70.9	0	71.2	0	71.1
1	72.7	1	73.4	1	72.5
2.5	78.2	2.5	77.1	2.5	82.3
5.0	81.2	5.0	79.7	5.0	86.9
10	83.6	10	84.0	10	89.2

T_m measurements were done in 3 separate experiments. The values given are the mean values. The SD in T_m measurements was <1 °C. The molar concentration of poly-L-lysine was calculated, using the average molecular weight of 50,000. At concentrations higher than 0.02 μM, DNA precipitated in the presence of poly-L-lysine.