Table 2.
Trilysine (Lys3)
|
Tetralysine (Lys4)
|
Pentalysine (Lys5)
|
|||
---|---|---|---|---|---|
Conc., μM | Tm,°C | Conc., μM | Tm, °C | Conc., μM | Tm, °C |
0 | 70.9 | 0 | 71.2 | 0 | 71.1 |
1 | 72.7 | 1 | 73.4 | 1 | 72.5 |
2.5 | 78.2 | 2.5 | 77.1 | 2.5 | 82.3 |
5.0 | 81.2 | 5.0 | 79.7 | 5.0 | 86.9 |
10 | 83.6 | 10 | 84.0 | 10 | 89.2 |
Tm measurements were done in 3 separate experiments. The values given are the mean values. The SD in Tm measurements was <1 °C. The molar concentration of poly-L-lysine was calculated, using the average molecular weight of 50,000. At concentrations higher than 0.02 μM, DNA precipitated in the presence of poly-L-lysine.