Figure 2.

NHE1 activity regulates attachment and spreading of CCL39 fibroblasts on FN. (A) CCL39 cells (a and b), EIPA-treated CCL39 cells (c and d), PS120 cells (e and f), and PS120N cells (g and h) were plated on FN-coated dishes. After 20 min, cells attached to FN (a, c, e, and g) and unattached cells in the medium (b, d, f, and h) were photographed. Bar, 30 μm. (B) Cells were plated on FN-coated dishes and photographed at 60 min. Adhesion and spreading of EIPA-treated CCL39 cells (b) were delayed compared with that in NHE1-expressing CCL39 cells (a) and in PS120N cells (d). (c) After 60 min, many PS120 cells had still not attached. Bar, 10 μm.