NHE1 activity regulates attachment and spreading of Ltk− fibroblasts on FN. (A) Ltk− cells (a and b), EIPA-treated Ltk− cells (c and d), and LAP1 cells (e and f) were plated on FN-coated dishes. After 20 min, cells attached (a, c, and e) and unattached (b, d, and f) to FN were photographed. Bar, 30 μm. (B) Cells were plated on FN-coated dishes, and attached cells were photographed at 60 min. Bar, 20 μm.