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. 1998 Aug;9(8):2287–2303. doi: 10.1091/mbc.9.8.2287

Figure 5.

Figure 5

NHE1 activity regulates the assembly of focal adhesions. Cells plated on FN-coated coverslips in SFM were fixed at 2 h and stained with anti-paxillin or fixed at 16 h and stained with anti-vinculin antibodies to visualize focal adhesions. EIPA-treated cells were pretreated with EIPA for 15 min before plating and allowed to attach on coverslips in the presence of EIPA. (A) Confocal images of paxillin after 2 h on FN in CCL39 cells (a), EIPA-pretreated CCL39 cells (b), PS120 cells (c), and PS120N cells (d). Bar, 1.5 μm. (B) Immunostaining for vinculin after 16 h on FN in CCL39 cells (a), EIPA-pretreated CCL39 cells (b), PS120 cells (c), and PS120N cells (d). Bar, 4 μm.