Figure 7.

Expression of vinculin and paxillin is regulated in PS120 cells. (A) Expression of α5 and β1 integrins is shown. Biotin-labeled lysates obtained from CCL39, PS120, and PS120N cells were incubated with antibodies to α5 and β1 integrins. Immunoprecipitated integrins were detected by streptavidin-horseradish peroxidase. (B) Expression of vinculin, paxillin, and actin was determined by immunoblotting whole-cell lysates (50 μg). Expression of FAK and c-Src was determined by immunoblotting immunoprecipitates of whole-cell lysates (250 μg).