Table 1.
Secondary structure analysis (infrared) for the free HSA and its polyamine analogue complexes in H20 at pH 7.2 (the mean deviation was ± 1.0 % to ± 2.0 % for the free protein and its polyamine complexes).
| Amide I components (cm−1) | Free HSA (%) 0.25 mM | HSA-333 (%) 1 mM | HSA-BE333 (%) 1 mM | HSA-BE3333 (%) 1 mM |
|---|---|---|---|---|
| 1692-1680 β-anti | 7 ± 1 | 4 ± 1 | 6 ± 1 | 3 ± 1 |
| 1680-1660 turn | 14 ± 1 | 15 ± 2 | 15 ± 2 | 18 ± 1 |
| 1660-1650 α-helix | 55 ± 1 | 51 ± 1 | 50 ± 1 | 43± 1 |
| 1648-1641 random coil | 7 ± 1 | - | - | - |
| 1640-1610 β-sheet | 17 ± 1 | 30 ± 1 | 29 ± 1 | 36 ± 1 |